Table 3. Comparative genomic analysis of the outbreak strains with the reference strains and the local strain CDC228^§.

Region	Location	Gene	Nucleotide Change (amino acid substitution)
			DG01_2011	CDC228	Outbreak strains
ITR	518	inverted terminal repeat	Insertion of T	—	—
E1A	815	28 kDa protein	—	—	T→G (W→L)
	1444		—	—	A→G
IVa2	4133	—	—	—	C→T
E2B	5517	DNA polymerase	G→C (D→E)	—	—
	7494		—	—	G→T (L→I)
	9168	preterminal DNA-binding protein	—	—	C→T&
	9327		—	—	G→T&
L1	10698–10709	Virus-associated RNA II	—	—	Deletion of GTTGCGGTGTGC
	10734		—	—	G→A
	10816		—	Insertion of T	Insertion of T
	10830		G→A	—	—
L2	14594	Penton	T→C (V→A)	—	—
	14854		—	—	A→G (N→D)
L3	17871	Protein VI precursor	—	—	C→G (P→A)
L4	24298	hexon-assembly associated protein	C→T&	—	—
	24904		—	—	T→C&
E3	27799	16.1 kDa protein	—	—	C→T (P→S)
Non-coding Region	29950	—	—	—	Insertion of T
E4	32450	E4	—	—	T→C (N→D)
	33155	32 kDa protein	G→T (P→Q)	—	—
Non-coding Region	34904	—	—	—	G→A
ITR	35125	inverted terminal repeat	—	—	C→A

“–” means no change or not applicable.

^§The reference genomes include the HAdV-7 strains DG01_2011 (KC440171) and 0901HZ/ShX/2009 (JF800905). Outbreak strains mean the nine HAdV-7 outbreak strains.

^&Synonymous mutation.