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. 2015 Sep 4;6:919. doi: 10.3389/fmicb.2015.00919

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Copyright © 2015 Mota, Alves, Carneiro, Silva, Brown, Istel, Kuchler, Sampaio, Casal, Henriques and Paiva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of phagocytosis of C. glabrata HTL, fps1, and ady2a cells by flow cytometry after 30 min of incubation at 37 °C with macrophages. Before the phagocytosis assay yeast cells were grown in two different conditions: RPMI pH 5.0 and RPMI 0.5% acetic acid at pH 5.0. Graph (A) represents the percentage of macrophages with yeast cells internalized, (B) shows the percentage of macrophages with yeast cells adhered to their surface, (C) corresponds to the percentage of macrophages with yeast cells internalized and adhered, and (D) displays the percentage of phagocytosis, resulting from the sum of (A) and (C). Error bars represent SD. ^∗, ^∗∗, and ^∗∗∗ means that results are statistically significant (p < 0.05), (p < 0.01), and (p < 0.001), respectively.