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. 2015 Sep 4;6:919. doi: 10.3389/fmicb.2015.00919

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Copyright © 2015 Mota, Alves, Carneiro, Silva, Brown, Istel, Kuchler, Sampaio, Casal, Henriques and Paiva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of the interaction of C. glabrata HTL, fps1, and ady2a cells with macrophages. (A) Percentage of C. glabrata killing by macrophages evaluated by CFU and (B) the TNF-α concentration released by macrophages infected with C. glabrata strains in pg/ml, Ctl1 and Ctl2 corresponds to the macrophages control and incubated with LPS, respectively. The results were assessed at 3 and 18 h post-infection (white and grey bars, respectively). Before the phagocytosis assay yeast cells were grown in two different conditions: RPMI pH 5.0 and RPMI 0.5% acetic acid at pH 5.0. Error bars represent SD. ^∗, ^∗∗, and ^∗∗∗ means that results are statistically significant (p < 0.05), (p < 0.01), and (p < 0.001), respectively, when comparing 3 with 18 h of incubation. Other comparisons are indicated by a bar.