Fig. 3.

Enhanced T_h17 response in Slamf6 ^−/− mice during C. rodentium-induced colitis. WT and Slamf6 ^−/− mice were infected by oral gavage of 2×10⁹ C. rodentium bacteria. At day 10 post-infection, leukocytes were isolated from the colon lamina propria. The percentage of (A) IL-17A⁺ and (B) IFNγ⁺ lymphocytes is represented as a percentage of total CD45⁺ CD4⁺ lymphocytes. (C) Representative dot plots of intracellular staining for IL-17A and IFNγ obtained from WT and Slamf6 ^−/− mice. Isolated CD4⁺ splenocytes were cultured o/n in the presence of plate-bound anti-CD3 antibody. The amount of (D) IL-17A and (E) IFNγ cytokines in the supernatant of these cultures is represented. WT and Slamf6 ^−/− CD45RB^hi CD4⁺ splenocytes were transferred into Rag ^−/− mice. The percentage of (F) IL-17A⁺ and (G) IFNγ⁺ lymphocytes is represented as a percentage of total CD45⁺ CD4⁺ lymphocytes. (H) Representative dot plot of intracellular staining for IL-17A and IFNγ obtained from Rag ^−/− mice in which WT T cells were transferred.