Fig. 5.

Reduced lamina propria inflammation during C. rodentium-induced colitis of Slamf6 ^−/− Rag ^−/− mice. Rag ^−/− and Slamf6 ^−/− Rag ^−/− mice were infected by oral gavage of 2×10⁹ C. rodentium bacteria. Uninfected Rag ^−/− mice are represented as controls. Animals were sacrificed on day 6 post-infection for analysis. (A) Quantification of flow cytometric analysis of isolated lamina propria macrophages. The total number of CD11c^hi MHC-II⁺ CD11b⁺ CD103⁻ Ly6G⁻ CD45⁺ cells and the percentage of CD11c^hi MHC-II⁺ CD11b⁺ CD103⁻ cells relative to the total pool of Ly6G⁻ CD45⁺ lamina propria cells are represented. (B) Quantification of flow cytometric analysis of isolated lamina propria neutrophils. The total number of CD11b⁺ Ly6G⁺ CD45⁺ cells and the percentage of CD11b⁺ Ly6G⁺ relative to the total pool of CD45⁺ lamina propria cells are represented. (C) Quantitative PCR relative expression of IL-12(p70) in colon homogenates of uninfected Rag ^−/− and Slamf6 ^−/− Rag ^−/− mice and 6 days after infection with C. rodentium.