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. 2015 Sep 3;5:30. doi: 10.1186/s13395-015-0055-5

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© Domi et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — MABs can synthesize laminin chains α4 and α5 but not α1. a Cultures of MABs differentiated in myotubes (MyHC positive) do not show staining with anti-laminin α1 chain antibody. DAPI staining identifies nuclei. a′ is the same image as in (a), without MyHC staining. Scale bar = 30 μm. b Cultures of MABs differentiated in myotubes show positive staining for laminin α4. DAPI staining identifies nuclei. Scale bar = 30 μm. c Cultures of MABs differentiated in myotubes show positive staining for laminin α5. DAPI staining identifies nuclei. Scale bar = 30 μm