Skip to main content
. 2015 Apr 16;24(14):3971–3981. doi: 10.1093/hmg/ddv135

Figure 1.

Figure 1.

Hsc70 activity and tau are required to stimulate tubulin assembly in vitro. (A) Polymerization of rhodamine-labeled tubulin in Xenopus oocyte extracts in the presence of WT tau or P301L tau and active or inactive Hsc70. Quantification of tubulin intensity, *P < 0.05, n = 10 one-way ANOVA (P = 0.0001, F = 7.0746 with Tukey's post-hoc test for pairwise comparisons). Scale bar is 30 µm. (B) Representative western blot showing polymerization of 5 µg purified bovine tubulin in the presence of active or inactive Hsc70 with and without tau. S, supernatant, P, pellet. Error bars are ± SEM n = 4. Data were tested by one-way ANOVA (P = 0.0100, F = 5.945 with Tukey's post-hoc test for pairwise comparisons, *P < 0.05).