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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Arthritis Rheumatol. 2014 Oct;66(10):2706–2715. doi: 10.1002/art.38754

Figure 2. ACPA production by RA plasmablasts.

Figure 2

(A) Fluorescence-activated gating strategy for single-cell sorting IgG-secreting plasmablasts from peripheral blood mononuclear cells of individuals with RA. Plasmablasts were first gated on live, single PBMCs and then gated on surface staining for IgG+ plasmablasts, as shown. SSC, side scatter (B) Antigen array screening of the binding to citrullinated peptides of antibodies produced by bulk cultured plasmablasts from patients with psoriatic arthritis (PsA), with anti-CCP+, or with anti-CCP− RA. Antibody-binding values were normalized as described in the methods; blue represents no reactivity, yellow moderate reactivity, and orange-red high reactivity. Cit, citrullinated; Cfc, citrullinated filaggrin peptide cyclic; Fib, fibrinogen; H2A, histone 2A; HSP 60, heat shock protein 60.