TABLE 1.
Validated O. tsutsugamushi F-box Anks and their paralogs
| F-box Ank | Paralog(s) (% similarity to F-box of validated F-box-containing Ank)a |
|---|---|
| Ank1_02 | Ank1_01 (95) |
| Ank2 | Single copy |
| Ank3_08b | Ank3_01 (100); Ank3_04 (100); Ank3_05 (no F-box)c; Ank3_06 (no F-box); Ank3_09 (no F-box); Ank3_11 (100); Ank3_13 (100); Ank3_15 (86) |
| Ank4_01 | Ank4_02 (100) |
| Ank5_01 | Ank5_02 (100), Ank5_03 (100) |
| Ank6_02 | Ank6_01 (100), Ank6_03 (100), Ank6_04 (100) |
| Ank8 | Single copy |
| Ank9 | Single copy |
| Ank10_01 | Ank10_02 (100) |
| Ank12_01 | Ank12_02 (100) |
| Ank13 | Single copy |
| Ank14 | Single copy |
| Ank15b | Single copy |
| Ank16 | Single copy |
| Ank17b | Single copy |
| Ank20 | Single copy |
a
Pseudogenes were excluded. Similarity is based on sequence comparison between the paralog's analogous F-box residues and the F-box of confirmed F-box-containing Anks, as designated in Fig. 7 for reference residues 1 to 21.
b
F-box-containing Ank that exhibited weak interaction with GST-SKP1 in coprecipitation assays.
c
Ank3 paralogs with the “no F-box” designation do not possess F-boxes.