FIG 8.

Comparison of cell survival of wild type and spoT mutant in response to U in the presence or absence of glucose. Strains were untreated (no U; black bars) or exposed to 50 μM uranyl nitrate (gray bars) and either not supplemented or supplemented with 1% glucose in 50 mM PIPES buffer at pH 7.0 for 1 h, after which CFU/ml were counted (left axis). Red bars represent the fold change in CFU/ml counts between the U condition and the control (right axis). Error bars represent standard deviations from four biological replicates. Higher susceptibility of the mutant to U than of the wild type is highlighted with an asterisk (*, P < 0.01).