TABLE 2.

Select gene candidates with low U fitness

Gene	Annotation	LogFCa
		Uranium library 1	Uranium library 2	Uranium library 3	No-metal library	Cadmium library
CCNA_00080	LexA-like transcriptional repressor	−1.17*	−2.43**	−1.59**	−0.70	−0.31
CCNA_03858	secB, protein translocase subunit	−1.67**	−1.97**	−3.32**	−0.14	1.46**
CCNA_03624	cztA, sodium bicarbonate cotransporter	−1.79**	−1.54**	−2.58**	−0.14	−0.79*
CCNA_00285	Acetylglutamate kinase	−0.97*	−1.50**	−2.00**	−0.87	0.55
CCNA_01379	rsaFb, type I secretion outer membrane protein	−0.93*	−1.41**	−1.91**	−0.13	0.20
CCNA_03625	cztR, LysR family transcriptional regulator	−1.29**	−1.41**	−2.26**	−0.06	−0.70
CCNA_03498	MarR/EmrR family transcriptional regulator	−0.98*	−1.36**	−2.23**	−0.42	0.42
CCNA_01521	Hypothetical protein	−0.98**	−1.20**	−3.29**	−0.69	−0.12
CCNA_00290	Autotransporter protein	−0.98**	−1.04**	−1.74**	−0.54	−0.77**
CCNA_02552	clpS, Clp protease adaptor protein	−0.14	−1.39**	−1.99**	−0.54	1.19**
CCNA_02553	clpA, Clp protease ATP-binding subunit	0.21	−1.31**	−1.33**	−0.36	1.85**
CCNA_02140	fliM, flagellar motor switch protein	−1.14**	−1.03**	−0.58*	−0.90	−0.77**
CCNA_01067	rsaFa, type I secretion outer membrane protein	−0.24	−1.03**	−0.91**	−0.37	1.54**
CCNA_01061	rsaE, type I secretion adaptor protein RsaE	−0.06	−1.32**	0.23	0.03	2.46**
CCNA_01622	ppGpp hydrolase-synthetase RelA/SpoT	−0.37	−0.98**	−0.12	−0.25	−0.02

^aLog₂ fold change in normalized total insertions per gene compared to values in the master libraries. *, P < 0.05; **, P < 0.01.