FIG 5.

Impact of acetate concentration on acetate metabolism after glucose exhaustion. Cells were grown in M9 minimal medium containing glucose-acetate mixtures (15 mM glucose plus 0 to 128 mM acetate) as the substrate. Growth and expression of key acetate anabolism genes were measured after glucose exhaustion (GE). (A) Maximal growth rates after GE. (B) Maximal acetate consumption rates after GE. (C) Expression of acetate anabolic genes 90 min after glucose exhaustion, relative to levels at mid-exponential growth on glucose.