FIG 6.

In vitro-adenylated extended-length MccA variants are active and enter cells through both YejABEF and SbmA. (A) Wild-type MccA or 20- and 25-amino-acid-long peptides containing C-terminal MccA sequences were adenylated by MccB in vitro and products analyzed by MALDI-MS. (B) Adenylation products, in 10-μl reaction aliquots, were deposited onto cell lawns formed by wild-type E. coli or the indicated mutants along with several control antibiotics. The results of overnight growth at 37°C are shown. The plate shown is representative of one of three independent experiments.