TABLE 2.
Bioactivity of elongated MccA peptide adenylates
| Compound | MIC (μM) for E. coli straina: |
||
|---|---|---|---|
| BL21(DE3) | 0256 | 0193 | |
| MRTGNAD-AMP | 10 | >60 | 10 |
| MRTGNAD-AMP(ap)b | 2.5 | >60 | 1.25 |
| GMRTGNAD-AMP | 2.5 | >60 | 5 |
| GMRTGNAD-AMP(ap) | 0.3 | >60 | 0.3 |
| GGMRTGNAD-AMP | 2.5 | >60 | 5 |
| GGMRTGNAD-AMP(ap) | 0.3 | 60 | 0.3 |
| GGGMRTGNAD-AMP | 2.5 | >60 | 5 |
| GGGMRTGNAD-AMP(ap) | 0.3 | 60 | 0.3 |
| GGGGGGMRTGNAD-AMP | 5 | >60 | 5 |
| GGGGGGMRTGNAD-AMP(ap) | 1.3 | 60 | 0.6 |
a
Bioactivity was measured with serial 2-fold dilutions of each compound in the diffusion test on a lawn of the indicated E. coli cells. MIC values presented are the minimal concentration at which the inhibition zone was visible around a 10-μl aliquot of compound.
b
ap, aminopropyl.