FIG 3.

Inhibition of the glyoxylate shunt increases acetate dissimilation. Standing cultures of M. tuberculosis H37Rv were grown for 10 days on 0.25 mM heptadecanoic acid as the carbon substrate. To limit carbon flow through the glyoxylate shunt, the isocitrate lyase was chemically inhibited by 2, 5, or 10 mM itaconate. The impact on growth was detected via optical density of the culture (A), and the resulting acetate formation was measured enzymatically in the supernatant (B). The data represent one of three independent experiments.