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The role of Acs and the Pta-AckA pathway in acetate assimilation. To analyze acetate utilization in M. tuberculosis, the wild type (WT), ΔackA, Δpta, and Δacs mutant strains, and complemented strains were grown for 10 days under fast-growth conditions. Acetate at 10 mM was supplied as the sole carbon substrate to the minimal medium, and growth was determined via optical density. The data represent the mean from three independent experiments; error bars indicate the SD.
