Skip to main content
. Author manuscript; available in PMC: 2015 Sep 4.
Published in final edited form as: Biotechnol Bioeng. 2013 Dec 6;111(5):1028–1037. doi: 10.1002/bit.25153

Figure 5. Effect of aggregate size on microparticle incorporation.

Figure 5

Cell-microparticle aggregate size was controlled by incubation in different sized hydrogel microwell arrays. (A, B) Confocal images of MIN6 β-cells (green) and laminin-coated microparticles (red) formed in microwells with (A) 100 μm and (B) 200 μm. Scale bars represent 100 μm. (C) Quantification of the total number of microparticles incorporated per different size aggregates. * denotes p<0.05 using student’s t-test. (D) Percent of the total cross-sectional area of each aggregate, summed over 100 μm in the z-direction, consisting of microparticles. No significant difference was found in the fraction of the aggregate occupied by microparticles relative to the percent occupied by cells. Error bars represent standard error of the mean for n ≥ 35 aggregates measured of each size.