Fig 1. Activation of Hog1 MAPK during glucose starvation is dependent of Ssk1.

(A) Schematic diagram of the yeast Hog1 pathway—Osmotic stress can be transduced by Sho1 and Sln1 activation branches. Proteins important for Hog1 phosphorylation during glucose starvation are highlighted. (B) Wild-type (wt), ssk1Δ and sho1Δ strains were exponentially grown and subjected to 0.4 M NaCl for 5 min (+NaCl) or starved for glucose for 45 min (-Glc). Cells were collected at the indicated times and equal amounts of cell extracts were analyzed by SDS-PAGE and by immunoblotting using anti-phospho-p38 antibody. Glucose-6-phosphate dehydrogenase (G6pd) was probed as the loading control. Levels of phosphorylated Hog1 were normalized using G6pd levels and then the relative quantification was normalized by time 0.