Fig 8. Sense and antisense DMPK RNA levels in DM1 fetuses.

qRT-PCR were performed in heart (left panels) and in brain (right panels) using specific primers and standard curves established with a known number of plasmid molecules with the amplicons as described previously [32]. Levels of sense and antisense DMPK transcripts were reported on graphs using 18S as internal control, in arbitrary units (a.u.). Cont 18–25: samples from control fetuses aged between 18–25 weeks. DM1 16–20: samples from DM1 fetuses aged between 16–20 weeks. DM1 33: sample from a 33 weeks old DM1 fetus. The mean and SEM are represented respectively, as horizontal and vertical bars. (**, p<0.01, two-tailed Student’s t-test).