Fig 2. Pressure threshold ratios for Ec-MscS with single point mutations at the sites indicated, to the most conserved residue in the MscS Superfamily.

Error bars represent the standard error of the mean for a minimum of 4 patches from a minimum of two independent preps. The star indicates the construct that is statistically different from wild-type MscS as determined using a Student’s T-test (* = p < 0.05).