Table 1. Kinetic values for wildtype channel and mutants.
| Pressure Threshold Ratio (PS/PL) | Conductance (nS) | Rate of Inactivation (sec) | Open Dwell Time (msec) | |
|---|---|---|---|---|
| WT | 0.69±0.03 | 1.32±0.08 | ND1 | ND2 |
| L118F | 0.56±0.02 | 1.34±0.06 | ND1 | ND2 |
| F127E | 0.54±0.07 | 1.33±0.04 | ND1 | ND2 |
| A120S | 0.67±0.01 | 1.30±0.02 | 2.1±1.5 | ND2 |
| F127T | 0.59±0.03 | 1.36±0.08 | 3.0±1.8 | ND2 |
| S114I | 0.65±0.02 | 0.96±0.08 | 1.4±0.1 | 1.4±1.7 |
| S114T | 0.61±0.03 | 1.34±0.04 | 1.9±0.5 | 2.1±3.6 |
| L123F | 0.73±0.03 | 1.25±0.04 | ND1 | 0.8±0.9 |
| F127K | 0.77±0.03 | 1.33±0.04 | 0.6±0.2 | 2.0±2.9 |
All values have been calculated from at least 4 independent patches from at least two preps and the error is the standard deviation.
ND1: For some mutants the rate of inactivation was infinity meaning that in the time that the pressure was applied no inactivation was observed.
ND2: Some channels did not have discrete single channel openings because of long open dwell times, and thus the open dwell time for the channels were not calculated.