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. 2015 Sep 4;10(9):e0137468. doi: 10.1371/journal.pone.0137468

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© 2015 Milani et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — (A) Section with a portion of intestine and connective tissue. Inside the intestinal epithelium, among batiprismatic cells (bc), a strong proliferation of VASPH stained germ cells (arrow) is observed. Many germ cells (arrow; magnified in the inset) have passed the basal lamina (asterisk) to the connective tissue (c). Scale bar = 47.57 μm. (B) In the connective tissue, in proximity of the intestine, germ cells (arrow) surround acini full of eggs (two eggs are highlighted with a dashed oval; nu: nucleus). In the egg, small stained granules are scattered in the cytoplasm (inset: granule magnification). Scale bar = 47.62 μm (inset scale bar = 4.87 μm). (C) At the periphery of an acinus lumen (al), very early oocytes of about 10 μm show big stained spots (one oocyte is magnified in the inset). Scale bar = 10.54 μm. Red: VASPH staining; blue: nuclear staining.