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. 2015 Sep 4;10(9):e0137210. doi: 10.1371/journal.pone.0137210

Fig 5. KPT-185 targets multiple signaling pathways in MCL cells.

Fig 5

(A) After an 18-h treatment of KPT-185 (i.e., 50nM for Z138 and MINO, 100 nM for JVM2 and Jeko-1), Cyclin D1 and its downstream target phosphorylated Rb expression were analyzed by immunoblot. (B, C) After an 18-h treatment of KPT-185 (i.e., 50nM for Z138 and 100 nM for JVM2, MINO, and Jeko-1), PIM1 and p27KIP (B), phospho-S6, and phospho-4EBP1 (C) were analyzed by immunoblot. For c-Myc expression analysis, cells were treated with 500 nM KPT-185. α-tubulin was used as a loading control. The intensity, compared to that of α-tubulin or p-S6K / S6K, p-4E-BP1 / 4E-BP1 levels after background subtraction were obtained using ImageJ software. The results are representative of three independent experiments.