Fig 3. Anti-proliferative effects of pentamidine and suramin on T. brucei.

Cell proliferation/viability was assayed as described in Fig 1 with the exception of the use of (A) pentamidine and (B) suramin at variable concentrations. (C) Time dependency of anti-proliferative effects of pentamidine at variable concentrations (12.8 to 128 nM) was performed analogously to the experiments shown in Fig 1. Control samples contained 100 μl of fresh medium instead of a trypanocidal compound. Pentamidine concentrations used were: 0 nM (■), 12.8 nM (▲), 51.2 nM (▼), 64 nM (♦) and 128 nM (●). (D) Recovery test of T. brucei cells exposed to pentamidine was done in 24-well in the presence of pentamidine (12.8 nM to 128 nM). Pentamidine concentrations used were: 0 nM (■), 12.8 nM (▲), 51.2 nM (▼), 64 nM (♦) and 128 nM (●).The results depict the mean of three independent measurements (n = 3), *p<0.05.