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Specific activity of glycolytic enzymes in T. brucei cell extracts in the absence and presence of 10 mM ethyl pyruvate. (A) hexokinase, (B) phosphofructokinase and (C) pyruvate kinase. All cell extracts were pre-treated with ethyl pyruvate for 30 min before activity testing. All experiments were performed in triplicates (n = 3), * p<0.05.
