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. 2015 Sep 4;10(9):e0137230. doi: 10.1371/journal.pone.0137230

Fig 4. Optimum pH and inhibitory effect of L-arabino-1,4-lactone on Galf-ase activity of ORF1110 protein.

Fig 4

(A) The optimum pH of ORF1110 protein for Galf-ase activity was determined using buffers of sodium acetate-acetic acid (4–5.5, closed circle), potassium phosphate (5.5–7.5, open triangle) and MOPS (7.5–8, closed square). (B) Competitive inhibition of Galf-ase activity by L-arabino-1,4-lactone was analyzed with (open circle) or without (closed circle) the inhibitor. Each data point indicates the mean with standard deviation (error bars) from three experiments.