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. 2015 Aug 14;195(6):2852–2860. doi: 10.4049/jimmunol.1500936

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Copyright © 2015 The Authors

This is an open-access article distributed under the terms of the CC-BY 3.0 Unported license.

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FIGURE 3. — Basal HLMF contraction and Smad2/3 nuclear translocation are inhibited by LXA₄. (A) Representative images from an NFC donor showing the spontaneous contraction of HLMFs within a collagen gel and its suppression by LXA₄. (B) Constitutive HLMF contraction was significantly attenuated by LXA₄ (10⁻⁸ mol) in both NFC- (n = 6) and IPF-derived (n = 5) cells (two-way ANOVA corrected by Sidak multiple-comparison test; NFC, *p = 0.0009; IPF, ^#p = 0.0001). (C) Representative images from an IPF donor displaying total Smad2/3 expression within HLMFs. At baseline, the HLMFs have staining both in the nucleus and within the cytoplasm, which is reduced upon incubation with LXA₄ (10⁻⁸ mol) for 1 h. (D) The ratio of total Smad2/3 nuclear to whole-cell staining was assessed by measuring grayscale intensity. IPF-derived (n = 6) HLMFs demonstrated increased expression of total Smad2/3 within the nucleus in comparison with NFC donors (n = 6) (^#p = 0.043, Mann–Whitney U test). LXA₄ (10⁻⁸ mol) significantly attenuated the amount of Smad2/3 localized to the nucleus in both NFC- and IPF-derived cells (two-way ANOVA corrected by Sidak multiple-comparison test; NFC, *p = 0.0254; IPF, **p = 0.0006).