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. 2015 Aug 28;9:4979–4988. doi: 10.2147/DDDT.S87842

Figure 6.

Figure 6

mRNA and protein expression of TGF-β1, IL-1β, and COL1 in KFs.

Notes: After treatment with chymostatin (100 μmol/L or 1,000 μmol/L), captopril (100 μmol/L or 1,000 μmol/L), chymostatin (1,000 μmol/L) + captopril (1,000 μmol/L), or valsartan (10−5 mol/L, 10−6 mol/L, 10−7 mol/L) for 48 hours, the expression of TGF-β1, IL-1β, and COL1 were downregulated in all groups. Chymostatin (1,000 μmol/L), chymostatin (1,000 μmol/L) + captopril (1,000 μmol/L), and valsartan (10−5 mol/L) had a significant inhibitory effect on the expression of TGF-β1, IL-1β, and COL1 in KFs (P<0.05). (A) The mRNA expression was tested by QRT-PCR; (B and C) the protein expression was tested by Western blotting. All values are mean ± SD. *P<0.05, **P<0.01 represent the mRNA or protein expression compared to the KFs group.

Abbreviations: COL1, collagen I; IL-1β, interleukin-1β; KFs, keloid fibroblasts; QRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation; TGF-β1, transforming growth factor-β1.