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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2015 Aug 3;22(9):672–678. doi: 10.1038/nsmb.3064

Figure 5. M. tuberculosis secretes TNT into the macrophage cytosol.

Figure 5

(a, b) THP-1 macrophages were infected with gfp expressing strains of wt Mtb, Mtb ΔcpnT, Mtb ΔcpnT::cpnTH792N Q822K or Mtb ΔESX-1 (mc26230) at an MOI of 10:1. Representative images are shown 48 h post infection when macrophages were fixed, selectively or completely permeabilized with digitonin (a) or digitonin plus Triton X-100 (b), respectively, and stained with α-TNT or α-Ag85 antibody (red). Magnification: 100x; Scale bar: 5 μm. N = 50 cells from three independent experiments.