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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Immunol Methods. 2015 May 30;424:106–110. doi: 10.1016/j.jim.2015.05.008

Figure 2.

Figure 2

Ficolin-2 purification from bioreactor supernatants using GlcNAc-agarose. A, schematic for ficolin-2 purification. B, silver stain of 15 μl aliquots of fractions from purification. Arrows indicate the major ficolin-2 bamd C, upper, immunoblot of 5 μl aliquots of purification samples; lower, dot immunoblot of 100 μl of purification samples at the indicated dilution. The vertical black line marks where another portion of the same dot blot is displayed beside the other portion. Fractions: Pre, starting material; Post, flow-through from GlcNAc column; W1-6, washes 1-6; E1-6, elutions 1-6.