Figure 5.

Effect of UA + Res on TPA-induced inflammatory gene expression and inflammatory cell infiltration. Epidermal RNA samples were prepared from groups of female ICR mice (n=4–5/group) treated using the short-term protocol. RNA samples were then subjected to qRT-PCR analysis as described in Materials and Methods. Panel A, qRT-PCR analysis of IL-1α, IL-1β, IL-22, and Cox-2. mRNA levels of IL-1α, IL-1β, and IL-22 were normalized to 18S and the mRNA level of Cox-2 was normalized to GAPDH. Panel B, quantitative evaluation of the effect of UA, Res and UA + Res on the number of mast cells in the dermis 48 hrs after the last TPA treatment. Positive cells were counted per 200 mm². Panel C, quantitative analysis of the effect of UA, Res and UA + Res on the number of CD45 positive cells in the dermis 48 hrs after the last TPA treat. Positive cells were counted per 200 mm². The graphs in all cases represent means ± SEM of at least 3 independent experiments. *, p<0.05 when compared to acetone group; **, p<0.05 when compared to TPA group; †, p<0.05 when compared to UA + TPA group; ‡, p<0.05 when compared to Res + TPA group; and #, p<0.05 when compared to UA + TPA and Res + TPA group. The Mann-Whitney U test was used for statistical comparisons.