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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cancer Prev Res (Phila). 2015 Jun 22;8(9):817–825. doi: 10.1158/1940-6207.CAPR-15-0098

Figure 5.

Figure 5

Effect of UA + Res on TPA-induced inflammatory gene expression and inflammatory cell infiltration. Epidermal RNA samples were prepared from groups of female ICR mice (n=4–5/group) treated using the short-term protocol. RNA samples were then subjected to qRT-PCR analysis as described in Materials and Methods. Panel A, qRT-PCR analysis of IL-1α, IL-1β, IL-22, and Cox-2. mRNA levels of IL-1α, IL-1β, and IL-22 were normalized to 18S and the mRNA level of Cox-2 was normalized to GAPDH. Panel B, quantitative evaluation of the effect of UA, Res and UA + Res on the number of mast cells in the dermis 48 hrs after the last TPA treatment. Positive cells were counted per 200 mm2. Panel C, quantitative analysis of the effect of UA, Res and UA + Res on the number of CD45 positive cells in the dermis 48 hrs after the last TPA treat. Positive cells were counted per 200 mm2. The graphs in all cases represent means ± SEM of at least 3 independent experiments. *, p<0.05 when compared to acetone group; **, p<0.05 when compared to TPA group; †, p<0.05 when compared to UA + TPA group; ‡, p<0.05 when compared to Res + TPA group; and #, p<0.05 when compared to UA + TPA and Res + TPA group. The Mann-Whitney U test was used for statistical comparisons.

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