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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Insect Mol Biol. 2015 Jun 25;24(5):539–550. doi: 10.1111/imb.12180

Figure 1.

Figure 1

Expression and affinity purification of recombinant (r) Amblyomma americanum (Aam) IGFBP-rP1. Affinity purification fractions of rAamIGFBP-rP1 expressed in insect cells (A) and in Pichia pastoris (B) were resolved on 12.5% SDS-PAGE and visualized by silver or Coomassie brilliant blue staining. Western blotting analysis validating insect cell (C) and yeast (D) expression rAamIGFBP-rP1 using the antibody to the c-terminus hexa-histidine tag antibody. Please note in figure 1D: lanes marked day 3–5 = samples were taken at days 3, 4, and 5.