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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Insect Mol Biol. 2015 Jun 25;24(5):539–550. doi: 10.1111/imb.12180

Figure 4.

Figure 4

Figure 4

Western blotting analysis to validate native AamIGFBP-rP1 secretion into host and expression profile in tick tissues. (A) Western blot detection of the rAamIGFBP-rP1 using antibodies to Amblyomma americanum tick saliva proteins (TSP), I – antibodies to 48h fed female TSP, II – antibodies to 24h fed female TSP, and III – antibodies to 4 days fed male TSP. Western blot detection of native AamIGFBP-rP1 in A. americanum tick salivary gland protein extracts (B) and saliva (C) collected from different time feeding points using mono-specific anti-rAamIGFBP-rP1 antibody.