Table 5.
Fluorescent readings of separate samples of OPA in DMSO-reagent solution and blanks
| Time elapsed after addition of OPA |
Fluorescence reading of samplea |
Fraction of maximum reading |
Fluorescence reading of blank |
Fraction of maximum readingb |
|---|---|---|---|---|
| 20 min | 1747 | 84.1% | 4.951 | 7.94% |
| 40 min | 1781 | 85.7% | 8.860 | 14.2% |
| 60 min | 1891 | 91.0% | 13.89 | 22.3% |
| 80 min | 1924 | 92.6% | 17.31 | 27.8% |
| 100 min | 1965 | 94.6% | 20.46 | 32.8% |
| 2 h | 1974 | 95.0% | 23.91 | 38.4% |
| 3 h | 2059 | 99.1% | 31.00 | 49.7% |
| 4 h | 2056 | 98.8% | 32.61 | 52.3% |
| 5 h | 2078 | 100.0% | 38.46 | 61.7% |
| 6 h | 2022 | 97.3% | 41.56 | 66.7% |
| 7 h | 1994 | 96.0% | 43.42 | 69.7% |
| 23 h | 1270 | 61.1% | 60.60 | 97.2% |
| 26 h | 1046 | 50.3% | 62.32 | 100.0% |
a
Fluorescence readings of samples were not corrected for blank readings.
b
Maximum reading of blank for this 26 h period.