Skip to main content
. 2015 Sep 7;8(9):1385–1395. doi: 10.1016/j.molp.2015.04.012

Figure 1.

Figure 1

Pi04089 Contributes to P. infestans Virulence.

(A)Pi04089 expression is up-regulated at 24 and 48 h post infection (hpi) of potato plants with P. infestans.

(B) Increase in the area colonized by P. infestans following Agrobacterium-mediated expression of GFP-04089 in one half of a leaf compared with the expression of a GFP control.

(C) Graph shows a significant increase (p < 0.001, t test, as indicated by asterisks) in mean diameter of P. infestans lesions following Agrobacterium-mediated expression of GFP-04089 compared with the expression of a GFP control. Error bars are SE, and the graph represents the combined data from three biological reps (n = 84 per construct).

(D) Confocal projection of N. benthamiana leaf epidermal cells transiently expressing GFP-04089, showing that the fusion protein accumulates in the cytoplasm and nucleus. The inset is a magnified single optical section through the cell nucleus, showing that the effector fusion forms a distinct ring around the nucleolus. Scale bar represents 50 μm.