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. 2015 Aug 25;6:7978. doi: 10.1038/ncomms8978

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(a) Graphic representation of a nucleosome structure with H2A Thr-101 in red (adapted from 1KX4)51. (b) Reaction scheme for generation of GlcNAcylated H2A. Cys-101 is converted to dehydroalanine (Dha), which functions as a Michael acceptor for reaction with the GlcNAc-thiol, so replacing the ether linkage with a structurally analogous thioether. (c) Schematic representation of H2A/B dimer and nucleosome refolding (wt H2A in dark green and GlcNAcylated H2A in red). (d) SEC traces for the refolding of H2A/B dimer from wt (blue) and H2A T101GlcNAc (red) and wt H2B. (e) SEC traces for the octamer refolding from wt H3/H4 tetramers and H2A/B-wt (blue) or H2A/B-GlcNAc (red) dimers. (f) SDS–PAGE analysis of the main fractions of the marked peaks (original image shown in Supplementary Fig. 16).