Figure 2. yuc6-1D mutant has increased tolerance and decreased ROS accumulation to MV-induced oxidative stress.

(a) Shown are WT (Col-gl) and yuc6-1D plants that were grown on Murashige and Skoog (MS) agar plates containing indicated concentrations of MV for 2 weeks. (b) Fresh weight (means±s.e.) of these plants is shown (n=10). (c) Two-week-old plants grown on MS agar were untreated (−MV) or exposed to 10 μM MV for 4 h (+MV). Bars indicate H₂O₂ contents. Data represent means±s.e., n=3. (d) Fluorescence staining for H₂O₂ production. Five-day-old seedlings were untreated (−MV) or exposed to 10 μM +MV for 4 h and stained with H₂DCF-DA. The fluorescence and white light images of stained root tips are shown. (e) Histochemical staining for H₂O₂ production. Detached leaves of 2-week-old WT, yuc6-1D, 35S:YUC6, 35S:iaaL and yuc6-1DX35S:iaaL plants5,16 were untreated or treated with 10 μM MV for 4 h. H₂O₂ accumulation was visualized by DAB as a dark brown stain.