Figure 5. Cys-85 is required for the drought tolerance and low ROS content of YUC6 overexpression plants.

DR5:GUS (Col-0) plants (WT) were compared with two independent YUC6-OX transgenic lines (7-3 and 17-2), two independent YUC6-OX^C85S transgenic lines (2 and 3), one YUC6-OX^mNADP line and one NTRA-OX line in the same background. (a) Survival assay was performed exactly as described in Fig. 1a. (b) Assay of water loss rate was performed exactly as described in Fig. 1b. Data represent means±s.e., n=3. (c) H₂O₂ production in response to drought stress. Fourth and fifth rosette leaves were harvested after 6-d drought from the plants shown in a. The leaves were stained with DAB (1 mg ml⁻¹, pH 3.8) for 4 h and H₂O₂ was visualized as a dark brown colour. (d) Quantification of H₂O₂ accumulations. Aerial parts of 3-week-old plants were detached and air-dried for 4 h (+Dry) or immediately harvested without air-drying (−Dry). Data represent means±s.e. (n=3).