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. 2015 Aug 27;6:8052. doi: 10.1038/ncomms9052

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(a) Design of the cross-shaped DNA origami structure that was used for close packing. Stacking interactions between origamis were prevented by adding poly-T (four consecutive thymine bases) single-stranded DNA tails to all four edges. (b,c) AFM images of the close-packed cross-shaped DNA origami nanostructures. (d,e) Cross-sectional profiles along lines A–B (d) and C–D (e) in (c). (f) Successive HS-AFM images of close-packed cross-shaped origami and the defects therein. Images were obtained at a scan rate of 0.2 frames per s. The elapsed time is shown in each image. Details are seen in Supplementary Movie 5. Scale bars, 200 nm.