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. 2015 Aug 19;6:8088. doi: 10.1038/ncomms9088

Figure 5. Liquid demixing of LCD-containing proteins is dynamic and phase separated compartments can exchange its constituents.

Figure 5

(a) PARG-depleted U-2-OS cells were co-transfected with full-length GFP–EWS and the prion-like domains of EWS and FUS fused to RFP. Cells were laser microirradiated and time-lapse movie stills from the first 10 min after irradiation shown. Green arrows (upper panels) point to the recruitment of full-length GFP–EWS to DNA damage sites. Red arrows (middle panels) point to the redistribution of a prion-like domain-containing liquid droplet in the vicinity of the laser track. Note that the appearance of the distinct light-diffracting stripe at the laser microirradiated region is concomitant with the dissolution of the light-diffracting liquid droplet formed by the prion-like domains (lower panels). Prion domain containing droplets in distal regions of the nucleus and in the cytoplasm appeared stable during the period of observation. (b) PARG-depleted U-2-OS cells were transfected with full-length Tm-EWS. Cells were laser microirradiated and time-lapse movie stills from the first 10 min after irradiation shown. Red arrows (upper panels) point to the recruitment of Tm-EWS to DNA damage sites. Note that the full-length protein Tm-EWS dissolves into microdroplets at later time points. Scale bars, 10 μm.