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. 2015 Aug 27;6:8076. doi: 10.1038/ncomms9076

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(a) Cis-ATG-mediated current evoked by two-photon illumination (1 ms, 740 nm) in a CA1 pyramidal cell while bath applying 400 μM ATG. (b) 2P-evoked cis-ATG-mediated currents with illumination spot parked at 0.5, 2 and 4 μm away from spine head. Illumination duration was 1 ms, and the wavelength set at 740 nm. (inset) Enlarged view of distance-dependent ATG evoked responses. Box over traces illustrates the time window over which spatial dependence was estimated for isochronal amplitude plots in (c) (Scale bar 2 μm). This was chosen to correspond to the time point at which the largest current reached 75% of its amplitude. (c) Normalized isochronal plots for six cells, with the average in black (half-width half-maximum=2.0 μm, red dotted lines). Error bars indicate s.e.m.