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. 2015 Aug 26;6:8093. doi: 10.1038/ncomms9093

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Immunoblot analysis of the MAPK and PI3K/AKT pathways reveals differential regulation of key proteins. Immunoblot analysis of protein lysates from eight murine uncultured melanoma samples: four from NRAS and four from NRAS-ΔPTEN. Western blot analyses were performed between three and six times, depending on the antibody with similar results.