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. 2015 Aug 26;6:8093. doi: 10.1038/ncomms9093

Figure 8. β-catenin induces CAV1 through miR-199a and miR-203.

Figure 8

(a) miR-199a-5p and miR-203 gene expression in NRAS and NRAS-ΔPTEN mouse tumour samples from miRNA expression arrays. (b) CAV1 mRNA expression post transfection with miRNA mimics for miR-199a and miR-203 in human Hs944T melanoma cells (fc, fold change). CAV1 knockdown after miRNA mimics overexpression from whole-cell protein lysates in duplicates was validated through western blotting (below). Statistical significance was determined by Mann–Whitney test. (c) miR-199a-5p and miR-203 gene expression in Hs944T after overexpression with miR mimic 199a-5p and 203, respectively (fold change). Error bars represent s.d. **P-value <0.01. Statistical significance was determined by Mann–Whitney test. (d) miRNA levels as determined by quantitative reverse transcriptase–PCR (fold change) of mir-203 and mir-199a in Hs944T post siRNA-mediated knockdown of β-catenin (siBCAT) or conversely overexpression of BCAT. Statistical significance was determined by Mann–Whitney test. (e) CAV1 mRNA levels after either siBCAT or BCAT overexpression in Hs944T cells. Whole-cell protein lysates were analysed via western blot (see below). Error bars represent s.d. *P-value <0.05, **P-value <0.01 and ***P-value <0.001. Statistical significance was determined by Mann–Whitney test. Experiments were performed in biological triplicates.