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. 2015 Aug 26;6:8099. doi: 10.1038/ncomms9099

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(a) The loss of HA-EmrE-C+ co-expressed with either HA-EmrE_{locked_in} or HA-EmrE_{locked_out} after protein synthesis has been stopped at time zero. HA-EmrE –C+ was visualized by western blotting for the penta-histidine epitope. (b) The expression of HA-EmrE and HA-EmrE-C+ in wild type and FtsH knockout cells. The protein was visualized by western blotting using the HA epitope.