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. 2015 Aug 26;6:8128. doi: 10.1038/ncomms9128

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(a) MDCK cells expressing JAM-A shRNAs under a doxycycline-regulated promoter were transduced with lentiviral vectors expressing murine Flag-tagged JAM-A (mJAM-A). Cells were grown in 3D collagen gels for 6–8 days and stained as indicated. Size bars, 10 μm. It is noteworthy that JAM-A knockdown results in a multilumenal phenotype, and that single lumen formation is restored on expression of murine JAM-A. (b) Statistical analysis of lumen formation in JAM-A knockdown cells transduced with either empty vector or murine Flag-JAM-A. Quantification of data shown in this figure was performed using one-way ANOVA with Tukey's post-hoc test, with three independent experiments in each condition, and is presented as means±s.e.m.; ns, not significant, ***P<0.001.