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. 2015 Sep 1;35(9):698–709. doi: 10.1089/jir.2014.0211

FIG. 1.

FIG. 1.

Interferon (IFN)-α induces downregulation of miR-221-3p in dendritic cells (DCs). (A) Heat map representing mean fold change in expression of 113 miRNAs (84 miRNAs profiled using Human miFinder miScript miRNA qPCR Array, SA Biosciences plus 29 miRNAs whose qPCR primers were designed according to similar strategy as miFinder array) in myeloid dendritic cells (mDCs) (n=2) upon 24 h IFN-α treatment. Asterisk (*) indicates miRNA with greater than 1.5-fold upregulation/downregulation in both biological replicates. (B) Bar graph indicating mean fold change in expression of 7 miRNAs (which were marked with asterisk in the previous graph) in mDCs (n=3). (C) Bar graph indicating mean fold change in expression of same 7 miRNAs in plasmacytoid dendritic cells (pDCs) (n=3) upon 24 h IFN-α treatment. (D) Out of the remaining 116 miRNAs profiled in pDCs, 16 miRNAs showed significant downregulation upon 24 h IFN-α treatment. Bar graph indicates the mean fold-downregulation of those 16 miRNAs. (E) Bar graph indicating mean fold change in expression of miR-221 in B cells upon 24 h IFN-α treatment. (F) Bar graph indicating the kinetics of IFN-α-induced downregulation of miR-221 in mDCs. P values were calculated using Student's t test (*P<0.05; **P<0.01). Error bars represent standard deviation (n=3). Color images available online at www.liebertpub.com/jir