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. 2015 Sep 1;35(9):698–709. doi: 10.1089/jir.2014.0211

FIG. 3.

FIG. 3.

Role of STAT3 in IFN-α-induced miR-221 downregulation. (A) Freshly isolated mDCs were rested for 1 h followed by 15-min IFN-α treatment. After treatment, pSTAT1 and pSTAT3 levels were measured by flow cytometry. The graph shows control mDCs (no IFN-α treatment) in dark gray histogram and IFN-α-treated mDCs in dashed lines histogram. Black histogram shows mDCs stained with isotype (IgG-2a κ) control antibody. (B) Bar graph indicating mean fold change in miR-221 expression in mDCs pre-treated with STAT3 inhibitor, BP-1-102 (2 h) before exposing them to IFN-α (24 h). The graph shows 4 bars, white: untreated with BP-1-102, untreated with IFN-α; black: untreated with BP-1-102, but treated with IFN-α; stripes: treated with BP-1-102, but untreated with IFN-α; gray: treated with BP-1-102, treated with IFN-α. P values were calculated using Student's t test (**P<0.01; ***P<0.001). Error bars represent standard deviation (n=3).