Fig. 6.

The MED8 subunit plays a role in sugar responsive growth and gene expression. a Sugar hypersensitive dark development of Col, hsr8-1, med8hsr8-1 and med8 mutants. Seedlings were grown vertically in the dark for 14 days on MS medium containing 1 % Glucose. b and c Quantitative Real-time PCR analysis of mRNA levels of cell wall modifying encoding genes PME17 and AtPME41 in Col, hsr8-1, med8hsr8-1 and med8. Seedlings are grown as described in (a) above. Errors bars represent SD from three biological replicates. **, p < 0.01 comparing Col to hsr8-1, and med8 hsr8-1 to med8 (Student’s t- test). Relative transcript levels (RTL) were calculated relative to the transcript level of the reference gene TUB6 (At5g12250). d to f Quantitative Real-time PCR analysis of BAM, APL3, and CHS mRNA levels in Col, med8, pft1-2 and the double mutant med8pft1-2 in response to glucose. Seedlings were grown on MS medium supplemented with 0.5 % glucose in constant light. After 7 days, the seedlings were transferred to glucose-free liquid MS medium for 24 h and then treated for 6 h with 3 % Glucose. Errors bars represent SD from three biological replicates. **, p < 0.01 comparing Col to med8 and pft1-2 to med8 pft1-2 (D); **, p < 0.01 comparing pft1-2 to med8 pft1-2 (E); **, p < 0.01 comparing Col to med8 and pft1-2 to med8 pft1-2 (F) (Student’s t- test). Relative transcript levels (RTL) were calculated using transcript levels of the reference gene TUB6 (At5g12250)