Table 3.

Most consistently changed miRNAs in AUD

Symbol	RNA-seq							Microarray
	miRBase acc#	miRBase family	MiRBase fam acc #	Ctrl RPM	AUD RPM	% Δ AUD	P-value	% Δ AUD	P-value
Tier A: RNA-seq p < 0.05, Array p < 0.1, consistent % Δ AUD
mir-96	MI0000098	mir-96	MIPF0000072	4.6	11.3	142.6	0.031	15.0	0.059
mir-320b-1	MI0003776	mir-320	MIPF0000163	61.0	144.6	137.1	0.004	22.2	0.071
mir-1976	MI0009986	mir-1976	MIPF0001633	4.1	9.9	138.1	0.039	24.1	0.081
Tier B: RNA-seq p < 0.05, consistent % Δ AUD
mir-24-1	MI0000080	mir-24	MIPF0000041	31.3	90.5	189.4	0.008	6.2	0.338
mir-30a	MI0000088	mir-30	MIPF0000005	108.2	209.9	94.0	0.014	5.1	0.860
mir-92b	MI0003560	mir-25	MIPF0000013	7141.9	4553.8	−36.2	0.016	−8.2	0.312
miR-96-5p	MIMAT0000095	mir-96	MIPF0000072	111.7	252.0	125.5	0.018	8.0	0.455
mir-127	MI0000472	mir-127	MIPF0000080	68.6	158.9	131.5	0.021	8.4	0.455
mir-136	MI0000475	mir-136	MIPF0000099	4.4	9.7	121.2	0.035	1.5	0.760
miR-301a-3p	MIMAT0000688	mir-130	MIPF0000034	205.9	85.1	−58.7	0.045	−2.4	0.878
mir-320b-2	MI0003839	mir-320	MIPF0000163	61.3	148.8	142.9	0.004	5.2	0.333
mir-421	MI0003685	mir-95	MIPF0000098	15.5	30.3	95.6	0.020	9.0	0.725
miR-660-5p	MIMAT0003338	mir-188	MIPF0000113	196.9	99.7	−49.4	0.028	−10.2	0.451
mir-671	MI0003760	mir-671	MIPF0000358	14.8	44.4	200.0	0.003	4.2	0.692
mir-3615	MI0016005	mir-3615	MIPF0001540	50.1	78.3	56.4	0.031	1.3	0.881
mir-3676	MI0016077			2.1	11.1	437.1	0.027	12.9	0.218

RNA-sequencing of human serum samples determined miRNAs with nominally significant alterations and trends in patients with AUDs. Comparison with microarray data subsequently identified changes that were consistent across platforms. MicroRNAs were classified into two tiers based on concurrence of both analyses. Where available, microRNA families for each miRNA are also listed. Note that these are nominal (uncorrected) p values

RPM normalized reads per million