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. 2015 Sep 5;16(1):683. doi: 10.1186/s12864-015-1879-4

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© García et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Quantification of the β-1,3-glucan synthase (GS) activity of mutants resistant to Caspofungin. Cell extracts from early log-phase cultures in YEPD medium at 30 °C of the wild-type (BY4741) and the 25 CAS resistant mutant strains were obtained, and used for enzymatic assays as described in Methods. It is shown the GS activity of the six mutants related to sphingolipid biosynthesis. Bars represent the percentage of GS specific activity in each mutant compared to that of the wild-type strain (100 % of GS specific activity). Data are the mean of three independent experiments. Error bars represent SD. Statistical analysis was carried out with a two-tailed, unpaired, Student’s t-test to analyze differences between the indicated mutants and the wild-type strain: **P ≤ 0.01; ***P ≤ 0.001